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Image Search Results
Journal: PLoS ONE
Article Title: The Treg-Specific Demethylated Region Stabilizes Foxp3 Expression Independently of NF-κB Signaling
doi: 10.1371/journal.pone.0088318
Figure Lengend Snippet: Dual luciferase assays were performed after transfecting reporter plasmids carrying the indicated inserts or an empty pGL3 vector (EV) into RLM-11 cells (T cell line), A20 cells (B cell line) or RAW 264.7 cells (macrophage cell line). Three hrs (RLM-11, A20) or 20 hrs (RAW 264.7) after transfection, cells were stimulated for 16 hrs with PMA/iono (RLM-11, A20) or for 14 hrs with LPS/IFN-γ (RAW 264.7), followed by measurement of luciferase activities (mean±SD, n = 3). Data are representative of two to four independent experiments.
Article Snippet: Transfections were carried out in 100 μl
Techniques: Luciferase, Plasmid Preparation, Transfection